IGF1 ELISA kit (Bovine)

SKU: SEA050Bo

Size: 96Tests

# of Times Cited in literature: 6

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IGF1

Target Full Name: Insulin Like Growth Factor 1

Alternative Names: IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

Target Species: Bovine

Uniprot: P07455

Gene ID: 281239

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Bovine IGF1 / Insulin Like Growth Factor 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.78-50ng/mL

Sensitivity: 0.28ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 (IGF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 (IGF1). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 (IGF1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 (IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 (IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 (IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 (IGF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;Endocrinology;

References Citing This Product: CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL

The use of herb mix and lucerne to increase growth rates of dairy heifers

RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES

Studies on quality attributes of skimmed colostrum powder

Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo bulls

Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes

Effect of betaine supplementation on growth performance, nutrient intake and expression of IGF-1 in Karan Fries heifers during thermal stress

Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …

INFLUENCE OF OMEGA–3 RICH FATTY ACID ENRICHED FEED ON BIOCHEMICAL MARKERS AND POST PARTUM REPRODUCTIVE PERFORMANCE OF …

Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves

Comparative study on genital status, energy balance and mineral content of swamp buffalo (Luit) heifers and cows under organized system of rearing in Assam?¡­

Effects of Concentrate and Bypass Fat Supplementations on Growth Performance, Blood Profile, and Rearing Cost of Feedlot Buffaloes