IFNb ELISA kit (Chicken)

SKU: SEA222Ga

Size: 96Tests

# of Times Cited in literature: 14

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: IFNb

Target Full Name: Interferon Beta

Alternative Names: IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast

Target Species: Chicken

Uniprot: Q90873

Gene ID: 554219

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Chicken IFNb / Interferon Beta

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 5.5pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Beta (IFNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Tumor immunity;Infection immunity;

References Citing This Product: Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo

ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages

The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection

Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation

Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens

Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation

Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion

Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway

Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I