Product Description
Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina is a protein-DNA interaction assay kit specially developed for Illumina high-throughput sequencing platforms. Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is a new method for studying protein-DNA interactions. By fusing Protein G with transposase, under the guidance of the antibody, it allows accurate targeting of the target protein and cutting of the DNA sequence near the target site. With an optimized reaction system and library preparation workflow, this kit offers multiple advantages over conventional chromatin immunoprecipitation followed by sequencing (ChlP-seq), including high success rate, excellent antibody compatibility, short turnaround time, and ease of use, making It is especially suitable for such fields as early embryonic development, stem cells, cancer, and epigenetics. All reagents in this kit have undergone strict quality control and function testing to ensure the optimal and repeatability of library preparation.
Applications
This product is intended for studying protein-DNA interactions in mammalian cells with 5,000 - 500,000 input cells. Yeast, plant tissues and cells should be treated before proceed ing with the assay using this kit.