Human Vitamin D 25-hydroxylase, CYP2R1 ELISA Kit

SKU: E3860Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol) (PubMed: 12867411, PubMed: 15465040, PubMed: 18511070). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives (PubMed: 15465040, PubMed: 18511070). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed: 12867411, PubMed: 15465040, PubMed: 18511070).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:CYP2R1

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.19ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Vitamin D 25-Hydroxylase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q6VVX0

MASS(Da):57,359

GeneID:120227

Synonyms:CYP2R1;Cytochrome P450 2R1;Vitamin D 25-hydroxylase

Gene names:CYP2R1

Standard solution:2400ng/L