Human VD3(Vitamin D3) ELISA Kit

SKU: ELK0811

Size:96T

Reactivity:Human

Alternative Names:Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

Assay Type:Competitive Inhibition

Sensitivity:2.07 ng/mL

Standard:400 ng/mL

Detection range:6.25-400 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Reproductive science;Nutrition metabolism;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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