Human Three-Prime Repair Exonuclease 1, TREX1 ELISA Kit

SKU: E6685Hu

Size:96 Tests-1 Kit

Background:Major cellular 3'-to-5' DNA exonuclease which digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini. Prevents cell-intrinsic initiation of autoimmunity. Acts by metabolizing DNA fragments from endogenous retroelements, including L1, LTR and SINE elements. Unless degraded, these DNA fragments accumulate in the cytosol and activate the IFN-stimulatory DNA (ISD) response and innate immune signaling. Prevents chronic ATM-dependent checkpoint activation, by processing ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates. Inefficiently degrades oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or upon absorption of UV light. During GZMA-mediated cell death, contributes to DNA damage in concert with NME1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:TREX1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.013ng/ml

Detection range:0.05-15ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Three-Prime Repair Exonuclease 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9NSU2

MASS(Da):33,212

GeneID:11277

Synonyms:3'-5' exonuclease TREX1;Deoxyribonuclease III;DNase III;Three-prime repair exonuclease 1;TREX 1;TREX1

Gene names:TREX1

Standard solution:16ng/ml