Human Tb4(Thymosin Beta 4) ELISA Kit

SKU: ELK4391

Size:96T

Reactivity:Human

Alternative Names:TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

Assay Type:Competitive Inhibition

Sensitivity:0.88 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Endocrinology;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P62328