Human TAC1(Tachykinin, Precursor 1) ELISA Kit

SKU: ELK5030

Size:96T

Reactivity:Human

Alternative Names:NK2; NKNA; NKA; TAC2; • PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide

Assay Type:Competitive Inhibition

Sensitivity:54.5 pg/mL

Standard:10000 pg/mL

Detection range:156.25-10000 pg/mL

Sample type:Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TAC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TAC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TAC1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P20366