Human sTNF-R2(soluble tumor necrosis factor receptor 2) ELISA Kit

SKU: ELK8640

Size:96T

Reactivity:Human

Alternative Names:sTNF-R2; soluble tumor necrosis factor receptor 2

Assay Type:Sandwich

Sensitivity:8.87 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Cancer

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTNF-R2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTNF-R2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTNF-R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTNF-R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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