Human Receptor-interacting Serine/Threonine-protein Kinase 2, PIPK2 ELISA Kit

SKU: E6617Hu

Size:96 Tests-1 Kit

Background:Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed: 26646181).

Research_area:Others

Applications:ELISA

Target_protein:RIPK2

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.028ng/ml

Detection range:0.05-30ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Receptor-Interacting Serine,Threonine-Protein Kinase 2

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O43353

MASS(Da):61,195

GeneID:8767

Synonyms:CARD-containing IL-1 beta ICE-kinase;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;Receptor-interacting protein 2;Receptor-interacting serine/threonine-protein kinase 2;RIP-2;RIPK 2;RIPK2;RIP-like-interacting CLARP kinase;Tyrosine-protein kinase RIPK2;

Gene names:RIPK2

Standard solution:32ng/ml