Human PI(Proinsulin) ELISA Kit

SKU: ELK5406

Size:96T

Reactivity:Human

Alternative Names:INS; Pro-Insulin; Preproinsulin

Assay Type:Competitive Inhibition

Sensitivity:5.18 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Endocrinology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI in the samples is then determined by comparing the OD of the samples to the standard curve.

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