Human N-Acylsphingosine Amidohydrolase 2,ASAH2 ELISA KIT

SKU: E6958Hu

Size:96 Tests-1 Kit

Background:Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10781606, PubMed:16229686, PubMed:26190575). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:15946935, PubMed:19345744, PubMed:24798654). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:11278489, PubMed:17475390). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16061940). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity). {ECO:0000250|UniProtKB:Q9JHE3, ECO:0000269|PubMed:10781606, ECO:0000269|PubMed:11278489, ECO:0000269|PubMed:15946935, ECO:0000269|PubMed:16061940, ECO:0000269|PubMed:16229686, ECO:0000269|PubMed:17475390, ECO:0000269|PubMed:19345744, ECO:0000269|PubMed:24798654, ECO:0000269|PubMed:26190575}.

Research_area:

Applications:ELISA

Target_protein:ASAH2

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.21ng/ml

Detection range:0.5-150ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:N-Acylsphingosine Amidohydrolase 2

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9NR71

MASS(Da):85516

GeneID:56624

Synonyms:ASAH2 HNAC1

Gene names:ASAH2

Standard solution:160ng/ml