Human Mitogen Activated Protein Kinase 9, MAPK9 ELISA Kit

SKU: E6432Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed: 22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity). MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Research_area:Signal Transduction; Cell Biology

Applications:ELISA

Target_protein:MAPK9

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.015ng/ml

Detection range:0.05-20ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Mitogen Activated Protein Kinase 9

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P45984

MASS(Da):48,139

GeneID:5601

Synonyms:c-Jun N-terminal kinase 2;JNK-55;MAP kinase 9;MAPK 9;MAPK9;Mitogen-activated protein kinase 9;SAPK1a;Stress-activated protein kinase 1a;Stress-activated protein kinase JNK2;

Gene names:MAPK9

Standard solution:24ng/ml