Human MCSFR(Colony Stimulating Factor Receptor, Macrophage) ELISA Kit

SKU: ELK5189

Size:96T

Reactivity:Human

Alternative Names:CD115; MCFSR; M-CSFR; CSF1R; CSF1-R; C-FMS; CSFR; FIM2; FMS; Colony Stimulating Factor 1 Receptor; Formerly McDonough Feline Sarcoma Viral(v-Fms)oncogene Homolog

Assay Type:Sandwich

Sensitivity:0.126 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;CD & Adhesion molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCSFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCSFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCSFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCSFR in the samples is then determined by comparing the OD of the samples to the standard curve.

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