Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit

SKU: ELK0702

Size:96T

Reactivity:Human

Alternative Names:P38 Mitogen-Activated Protein Kinase

Assay Type:Sandwich

Sensitivity:0.53 ng/mL

Standard:100 ng/ml

Detection range:1.57-100 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK in the samples is then determined by comparing the OD of the samples to the standard curve.

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