Human Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) ELISA Kit

SKU: AE36061HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q6GTX8

Abbreviation: LAIR1

Alternative Names: XXbac-BCX403H19.4; CD305; LAIR-1; leukocyte-associated Ig-like receptor 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.129 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LAIR1 in samples. An antibody specific for LAIR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAIR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAIR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAIR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: LAIR1 is a type I membrane protein with a leader sequence, an extracellular domain, a transmembrane domain, and a cytoplasmic region. LAIR-1 is a 32 kDa transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. LAIR-1 recruits SHP-1 and SHP-2 phosphatases upon activation, and cross-linking of the LAIR-1 antigen on natural killer (NK) cells results in strong inhibition of NK cell-mediated cytotoxicity. Although it is structurally related to human killer cell inhibitory receptors, LAIR-1 does not appear to recognize human leukocyte antigen (HLA) class I molecules and thus represents a novel HLA class I-independent mechanism of NK cell regulation.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).