Human Interleukin 10, IL-10 ELISA Kit

SKU: E0102Hu

Size:96 Tests-1 Kit

Background:Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed: 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed: 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed: 1940799, PubMed: 7512027, PubMed: 11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed: 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Research_area:Immunology; Cancer

Applications:ELISA

Target_protein:IL10

Reactivity:Human

Assay type:Sandwich

Sensitivity:2.59pg/ml

Detection range:5-1500pg/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Interleukin 10

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P22301

MASS(Da):20,517

GeneID:3586

Synonyms:CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

Gene names:IL10

Standard solution:1600pg/ml