Human Interleukin-1 Receptor-associated Kinase 1, IRAK1 ELISA Kit

SKU: E5505Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Research_area:Others

Applications:ELISA

Target_protein:IRAK1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.046ng/ml

Detection range:0.1-40ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Interleukin-1 Receptor-Associated Kinase 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P51617

MASS(Da):76,537

GeneID:3654

Synonyms:Interleukin-1 receptor-associated kinase 1;IRAK 1;IRAK1;IRAK-1

Gene names:IRAK1

Standard solution:48ng/ml