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GeneBio Systems

Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

E2069Hu

$1,025.92 CAD
$1,025.92 CAD
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Size:96 Tests-1 Kit

Background:Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed: 11846977, PubMed: 16846591, PubMed: 16979567, PubMed: 20049431, PubMed: 32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed: 16846591, PubMed: 16979567, PubMed: 20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed: 16154084, PubMed: 27302953, PubMed: 33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed: 16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed: 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed: 33440148).

Research_area:Others

Applications:ELISA

Target_protein:IRF3

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.054ng/ml

Detection range:0.1-30ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Interferon Regulatory Factor 3

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q14653

MASS(Da):47,219

GeneID:3661

Synonyms:Interferon regulatory factor 3;IRF 3;IRF3;IRF-3

Gene names:IRF3

Standard solution:32ng/ml

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