Human Homeobox Protein Nanog, NANOG ELISA Kit

SKU: E5432Hu

Size:96 Tests-1 Kit

Background:Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed: 25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Research_area:Stem Cells; Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:NANOG

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.44ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Homeobox Protein NANOG

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9H9S0

MASS(Da):34,620

GeneID:79923

Synonyms:hNanog;Homeobox protein NANOG;Homeobox transcription factor Nanog;NANOG

Gene names:NANOG

Standard solution:3200ng/L