Human Hexose-6-Phosphate Dehydrogenase;Glucose 1-Dehydrogenase,H6PD ELISA KIT

SKU: E5324Hu

Size:96 Tests-1 Kit

Background:Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes (By similarity). Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway (PubMed:12858176, PubMed:18628520, PubMed:23132696). In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway (By similarity). May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate (By similarity). The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum (PubMed:12858176, PubMed:18628520, PubMed:23132696). By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity (PubMed:18628520). {ECO:0000250|UniProtKB:Q8CFX1, ECO:0000269|PubMed:12858176, ECO:0000269|PubMed:18628520, ECO:0000269|PubMed:23132696}.

Research_area:

Applications:ELISA

Target_protein:H6PD

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.85ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:GDH;6PGL endoplasmic bifunctional protein [Includes: Glucose 1-dehydrogenase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O95479

MASS(Da):88893

GeneID:9563

Synonyms:H6PD GDH

Gene names:H6PD

Standard solution:4800ng/L