Human GSK3a(Glycogen Synthase Kinase 3 Alpha) ELISA Kit

SKU: ELK1707

Size:96T

Reactivity:Human

Alternative Names:Serine/threonine-protein kinase GSK3A

Assay Type:Sandwich

Sensitivity:0.05 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;Enzyme & Kinase;Metabolic pathway;Endocrinology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GSK3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GSK3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GSK3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GSK3a in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P49840