Human G Protein-coupled Receptor Kinase 5, GRK5 ELISA Kit

SKU: E5311Hu

Size:96 Tests-1 Kit

Background:Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).

Research_area:Signal Transduction

Applications:ELISA

Target_protein:GRK5

Reactivity:Human

Assay type:Sandwich

Sensitivity:10.14ng/L

Detection range:20-3800ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:G Protein-Coupled Receptor Kinase 5

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P34947

MASS(Da):67,787

GeneID:2869

Synonyms:G protein-coupled receptor kinase 5;G protein-coupled receptor kinase GRK5;GRK 5;GRK5

Gene names:GRK5

Standard solution:4000ng/L