Human E3 Ubiquitin-protein Ligase Dtx3L, DTX3L ELISA Kit

SKU: E7314Hu

Size:96 Tests-1 Kit

Background:E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed:12670957, PubMed:19818714, PubMed:26479788, PubMed:23230272). Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed:28525742). In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed:19818714). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed:19818714). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed:26479788). Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:24790097). In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed:24790097). In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation (PubMed:26479788).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:DTX3L

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.01ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:E3 ubiquitin-protein ligase DTX3L

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q8TDB6

MASS(Da):83,554

GeneID:151636

Synonyms:BBAP;B-lymphoma- and BAL-associated protein;DTX3L;E3 ubiquitin-protein ligase DTX3L;Protein deltex-3-like;Rhysin2;Rhysin-2;RING-type E3 ubiquitin transferase DTX3L;RNF143;

Gene names:DTX3L

Standard solution:3200ng/L