Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit

SKU: ELK5354

Size:96T

Reactivity:Human

Alternative Names:DHC1b; DHC2; DNCH2; DYH1B; hdhc11; Dynein heavy chain isotype 1B; Dynein heavy chain 11; Dynein cytoplasmic heavy chain 2; Cytoplasmic dynein 2 heavy chain

Assay Type:Sandwich

Sensitivity:0.122 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:Tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYNC2H1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYNC2H1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYNC2H1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYNC2H1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q8NCM8