Human Dermatan-sulfate epimerase (DSE) ELISA Kit

SKU: AE46591HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q9UL01

Abbreviation: DSE

Alternative Names: DSEPI; SART2; OTTHUMP00000017059|squamous cell carcinoma antigen recognized by T cells 2

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.114 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate DSE in samples. An antibody specific for DSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: DSE is a tumor-rejection antigen.Two transcript variants encoding the same protein have been found for this gene.Sequence analysis predicted that the 958-amino acid SART2 protein contains a signal peptide, 5 potential N-glycosylation sites, and 2 C-terminal hydrophobic potential transmembrane regions. Northern blot analysis revealed expression of a 4.0-kb transcript in all squamous cell carcinoma (SCC) cell lines and in some adenocarcinoma cell lines tested. Expression was detected in all normal tissues tested, with highest expression in kidney and ovary and lowest expression in colon, thymus, and brain. Immunofluorescence microscopy demonstrated intracellular expression of SART2 in Golgi, endoplasmic reticulum, and secretory vesicles, but not on the plasma membrane.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).