Human Delta (3,5)-Delta (2,4)-dienoyl-CoA isomerase, mitochondrial (ECH1) ELISA Kit

SKU: AE46242HU

Species Reactivity:Human (Homo sapiens)

UniProt:Q13011

Abbreviation:ECH1

Alternative Names:HPXEL; delta3;5-delta2;4-dienoyl-CoA isomerase|dienoyl-CoA isomerase|peroxisomal enoyl-CoA hydratase 1|peroxisomal enoyl-coenzyme A hydratase-like protein

Application:ELISA

Range:0.156-10 ng/mL

Sensitivity:0.058 ng/mL

Intra-AssayCV:?5.3%

Inter-AssayCV:?10.1%

Recovery:1.02

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate ECH1 in samples. An antibody specific for ECH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial is a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, ?3,5-?2,4-dienoyl-CoA isomerase, contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).