Human Cyclin-dependent kinase 16 (PCTK1) ELISA Kit

SKU: AE28334HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q00536

Abbreviation: PCTK1

Alternative Names: FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE; PCTK1; OTTHUMP00000023209|PCTAIRE protein kinase 1|PCTAIRE-motif protein kinase 1|serine/threonine-protein kinase

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 12.6 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate PCTK1 in samples. An antibody specific for PCTK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCTK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCTK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCTK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: CDK16 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Two transcript variants encoding the same protein have been found for this gene. The 3 PCTAIRE kinases are 65% identical to one another (80% within the catalytic domain). PCTAIRE1 is ubiquitously expressed with the highest levels detected in the brain and testis. Human PCTAIRE cDNAs do not complement yeast cdc28 mutants and show other differences, suggesting that PCTAIRE kinases have different cellular functions from those described for CDC2.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).