Human CRH(Corticotropin Releasing Hormone) ELISA Kit

SKU: ELK10412

Size:96T

Reactivity:Human

Alternative Names:CRF; Corticotropin Releasing Factor; Corticoliberin

Assay Type:Competitive Inhibition

Sensitivity:0.13 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P06850