Human Cholesterol 7-alpha-hydroxylase,CYP7A1 ELISA KIT

SKU: E5037Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:2384150, PubMed:21813643). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:2384150, PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124). Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643). {ECO:0000269|PubMed:11013305, ECO:0000269|PubMed:12077124, ECO:0000269|PubMed:19965590, ECO:0000269|PubMed:21813643, ECO:0000269|PubMed:2384150}.

Research_area:

Applications:ELISA

Target_protein:CYP7A1

Reactivity:Human

Assay type:Sandwich

Sensitivity:4.39ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cholesterol 7α-Hydroxylase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P22680

MASS(Da):57661

GeneID:1581

Synonyms:CYP7A1 CYP7

Gene names:CYP7A1

Standard solution:2400ng/L