Human CC16(Clara Cell Protein 16) Microsample ELISA Kit

SKU: ELK10305MS

Size:96T

Reactivity:Human

Alternative Names:SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

Assay Type:Sandwich

Sensitivity:1.11 ng/mL

Standard:200 ng/mL

Detection range:3.13-200 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, lung lavage fluid , cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Infection immunity;Cardiovascular biology;Pulmonology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.

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