Human B2-glycoprotein 1 (β2-GP1) antibody lgA ELISA Kit

SKU: ELK0937

Size:96T

Reactivity:Human

Alternative Names:APOH; BG; B2G1; B2GP1

Assay Type:Sandwich

Sensitivity:26.8 pg/mL

Standard:4000 pg/mL

Detection range:62.5-4000 pg/mL

Sample type:Serum, plasma and other biological fluids.

Assay length:3.5h

Research Area:Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human β2-GP1 lgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human β2-GP1 lgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human β2-GP1 lgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human β2-GP1 lgA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: