Human Aldolase (ALD) ELISA Kit

Human Aldolase (ALD) ELISA Kit

AE61797HU
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$1,360.00 CAD
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Species Reactivity:Human (Homo sapiens)

UniProt:P04075

Abbreviation:ALDOB

Alternative Names:RP11-490D19.1; ALDB; ALDO2; aldolase 2|aldolase B; fructose-bisphosphatase

Application:ELISA

Range:24.69-2000 pg/mL

Sensitivity:8.85 pg/mL

Intra-AssayCV:?3.9%

Inter-AssayCV:?6.3%

Recovery:0.84

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate ALDOB in samples. An antibody specific for ALDOB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALDOB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDOB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDOB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:Aldosterone is a steroid hormone with a molecular weight of 360.4. It is secreted by the glomerulosa zone of the adrenal gland and controls regulation of hydromineral metabolism. In the distal part of the nephron, aldosterone encourages reabsorption of Na and Cl- ions and secretion of K and H ions in blood vessel lumen. Thus there is an increase in extra-cellular osmolarity and water retention. Regulation of aldosterone secretion is linked to the body's water balance and brings three factors into play. These are the renin- angiotensin system, the plasmic Na /K ratio and, as a secondary factor, the ACTH. Physiologically, there seems to be circadian rythm for levels of aldosterone in the blood, which may be linked to changes in body posture. Aldosterone levels are higher for children than for adults, and increase during pregnancy.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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