Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

Human A Disintegrin And Metalloprotease 12 (ADAM12) ELISA Kit

AE23769HU
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Species Reactivity:Human (Homo sapiens)

UniProt:O43184

Abbreviation:ADAM12

Alternative Names:RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha)|meltrin alpha

Application:ELISA

Range:0.156-10 ng/mL

Sensitivity:0.057 ng/mL

Intra-AssayCV:?4.3%

Inter-AssayCV:?7.3%

Recovery:1.05

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate ADAM12 in samples. An antibody specific for ADAM12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAM12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:ADAM12 encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. Similarly to myogenin, a marker of early muscle differentiation, mouse meltrin-alpha is expressed in neonatal muscle and bone, and its expression increases dramatically in response to the induction of differentiation. Immunocytochemical localization and functional expression studies suggested that meltrin-alpha may be involved in myotube formation.

Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

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