Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit

SKU: ELK8402

Size:96T

Reactivity:Human

Alternative Names:8-isoprostane; 8-iso-PGF2α

Assay Type:Competitive Inhibition

Sensitivity:8 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;Pulmonology;Hepatology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-iso-PGF2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-iso-PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-iso-PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.

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