HSP70 Mouse mAb

SKU: EM1042

Size: 100μL

Source:Mouse

Applications:WB, IHC, IF

Reactivity:Human, Rat, Mouse

Dilution:WB 1:1,000-2,000 IF 1:100-200 IHC 1:200-500

Immunogen:Synthetic Peptide

Storage_stability:PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality:Monoclonal

Isotype:IgG1

Concentration:1mg/mL

Observed_band(KD):70kDa

Human_gene_id:3303

Human_swiss_prot_no:P0DMV8

Subcellular_location:Cytoplasm

Other_name:Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Background:The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.