HRP Conjugated AffiniPure Goat Anti-Human IgM

SKU: BA1077

Size: 0.25ml

Storage: At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.

Form: Concentrated, Liquid

Reactivity: Human

Applications: ELISA,WB

Application Details: Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)

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Specificity: This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.

Background: Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Immunogen: Human IgM (whole molecule).

Clonality: Polyclonal

Contents: 0.25 mg of HRP conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.

Purification: The antibody was purified from antisera by immunoaffinity chromatography.

Reconstitution:

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Host: Goat

Concentration: 1mg/ml

Conjugate: HRP

Cross Reactivity:

Isotype: IgM

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