Horse COL1(Collagen Type I) ELISA Kit

SKU: ELK8222

Size:96T

Reactivity:Horse

Alternative Names:COL1; COLI

Assay Type:Competitive Inhibition

Sensitivity:7.29 ng/mL

Standard:1500 ng/mL

Detection range:23.44-1500 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Developmental science;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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