Size: 
100 rxn (20 μl/rxn)
• Based on high effective HiScript II Reverse Transcriptase.
• Flexible options of RT primers for different applications.
• Ready-to-use SuperMix.
The HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR is designed for 2-step RT-qPCR. The 5× HiScript II Select qRT SuperMix contains Buffer, dNTPs, HiScript II Reverse Transcriptase, and RNase inhibitor. The Oligo (dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.
The HiScript II Q Select RT SuperMix for qPCR has been specially optimized for qPCR. The cDNA products are compatible for SYBR- or probe-basded qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).
|
|
R211 |
R212 |
R222 |
R223 |
R232 |
R233 |
|
Application |
|
|
|
|
|
|
|
RT-qPCR |
|
|
√ |
√ |
√ |
√ |
|
universal |
√ |
√ |
|
|
|
|
|
Features |
|
|
|
|
|
|
|
Ready-to use |
|
|
√ |
√ |
√ |
√ |
|
Long cDNA synthesis |
√ |
√ |
|
|
√ |
√ |
|
gDNA wiper |
|
√ |
|
√ |
|
√ |
|
Primers |
|
|
|
|
|
|
|
Oligo (dT)23VN/N6 Mix |
|
|
√ |
√ |
|
|
|
self-selection |
√ |
√ |
|
|
√ |
√ |
Table 1. Guidelines for choice of HiScript II two-step RT-PCR/qPCR series.