GeneBio Systems

GREM1 ELISA kit (Human)

Name: GREM1 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEC128Hu
Price: 1470.00 CAD
Availability: InStock

SKU:SEC128Hu

$1,470.00 CAD

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Quantity

Size: 96Tests

# of Times Cited in literature: 14

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: GREM1

Target Full Name: Gremlin 1

Alternative Names: PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

Target Species: Human

Uniprot: O60565

Gene ID: 26585

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human GREM1 / Gremlin 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 6.25-400ng/mL

Sensitivity: 2.35ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Urine, Tissue Homogenates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gremlin 1 (GREM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Gremlin 1 (GREM1). No significant cross-reactivity or interference between Gremlin 1 (GREM1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gremlin 1 (GREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gremlin 1 (GREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gremlin 1 (GREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gremlin 1 (GREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Tumor immunity;Developmental science;Bone metabolism;

References Citing This Product: Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension

Overexpression of Gremlin promotes non-small cell lung cancer progression

Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients

Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-termprognosis in suspected and proven acute heart failure patients

BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH

GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness

The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.

Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.

Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial …

Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells

Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation