Gel-PCR 2in1 Kit- HiPure DNA Kit

D211101

Gel-PCR 2in1 Kit-HiPure Gel DNA Mini Kit

D2111

CAT NO	PRODUCT NAME	SIZE
D211102	HiPure Gel DNA Mini Kit	100 preps
D211103	HiPure Gel DNA Mini Kit	250 preps

Introduction

Gel-PCR 2in1 Kit- HiPure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments between 60bp-10kbp from agarose gel
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤500mg
Recovery	≥80%
Elution volume	≥15μl
Time per run	≤20 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The Gel-PCR 2in1 Kit-HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Now, keep this one kit in your lab and use it for your gel extraction purification or your PCR or other enzymatic reaction products, it is your choice which application you need to use the kit for!

Comparing to four other leading brands (M is GeneBio-Magen's product)-see below. 

Advantages

• High recovery efficiency - ≥80% DNA recovery
• General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents	D211101	D211103
Number of Preps	50 Preps	250 Preps
Buffer GDP	50 ml	250 ml
Buffer DW2	20 ml	2 x 50 ml
Elution Buffer	6 ml	30 ml
HiPure DNA Mini Columns II	50	250
2 ml Collection Tubes	50	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.