GB-Amp™ InFluor™ Green qPCR Mix

SKU: P2092

GB-Amp™ InFluor™ Green qPCR Mix is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with InFluor™ Green. About InFluor™ Green-a nucleic acid (DNA and RNA) intercalating dye, which is fully compatible will all major real-time PCR (qPCR) systems, specifically, InFluor™ Green fluorescence can be read in the green channel of the real-time PCR thermal cyclers for quantitative PCR analyses. The InFluor™ does not inhibit PCR amplification. It supports melt curve analyses. Applications • Real-Time PCR • Real-Time PCR RT-PCR The system contains sufficient reagents suitable for 200 amplification reactions of 50 μl PCR system. Nuclease-Free Water is also provided for your convenience in setting up PCR reactions. Storage This reagent can be stored at 4°C for 2 months when stored protected from light. For longer storage, this reagent should be kept at -20°C and protected from light. Features • This reagent can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.). • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems. • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible amplification. Composition of our qPCR Mix (when diluted with your primers and samples) 100 mM KCl , 4 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Taq DNA Polymerase, 1x InFluor™ Green and other optimized buffer components. Detection • This reagent can be used in general detection devices, such as: LineGene (Bioer Technology co., ltd.) • This reagent can also be used in detection equipment using glass capillaries or passive reference, such as: LightCycler (Roche Molecular Systems) ABI PRISM® 7000, 7700, and 7900 (Applied Biosystems) Note: The passive reference mode of detectors should be set at“ROX”. Guidelines for preventing contamination of PCR reaction During PCR more than 10 million copies of template DNA are generated. Therefore, care must be taken to avoid contamination with other templates and amplicons that may be present in the laboratory environment. General recommendations to lower the risk of contamination are as follows: • Prepare your DNA sample, set up the PCR mixture, perform thermal cycling and analyze PCR products in separate areas. • Set up PCR mixtures in a laminar flow cabinet equipped with an UV lamp. • Wear fresh gloves for DNA purification and reaction set up. • Use reagent containers dedicated for PCR. Use positive displacement pipettes, or use pipette tips with aerosol filters to prepare DNA samples and perform PCR set up. • Always perform “no template control” (NTC) reactions to check for contamination Quality Control The absence of endodeoxyribonucleases, exodeoxyribonucl- eases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

Customer Reviews

Quality to price ratio can't be beat

We tried this product for qRT-PCR on a CFX96 and were blown away and the consistent, high quality data produced. This product compares to reagents that are much more expensive. Would strong recommend.

Great product!!

Tried the Kit and it work very well on our CFX Bio-rad instrument. Great price and a very helpfull and nice people to talk to for there help and technical advice, I recommand it for shure. Thanks

Amazing product!!

We first tried a sample they kindly gave us and were very happy to see that it worked as well as other more expensive brands. Since then we have purchased 100 ml. The customer service is also excellent, they are very understanding and knowledgeable.

SYBR works great

This SYBR Green works very well. We switched over to using this one because of the lower price and because we can't tell the difference compared to other more costly brands in terms of quality. We use it on a Biorad CFX but as far as I know it is quite versatile and can be used on many different instruments. Would highly recommend to try it out. Customer service is also excellent as well!

Great product, low price

I have used GeneBio Systems's SYBR Green qPCR mix numerous times and have always been impressed with the product quality, especially considering the price! The fact that it is a master mix makes qPCR prep easier, faster, and reduces the likelihood of pipetting errors. The SYBR green qPCR mix is also compatible with a variety of qPCR-associated instruments. Would highly recommend.