EZH2 CLIA kit (Human)

SKU: SCK073Hu

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: EZH2

Target Full Name: Enhancer Of Zeste Homolog 2

Alternative Names: ENX-1; KMT6; KMT6A; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6

Target Species: Human

Uniprot: Q15910

Gene ID: 2146

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human EZH2 / Enhancer Of Zeste Homolog 2

Method: Chemiluminescence

Detection principle: Double-antibody Sandwich

Detection range: 13.7-10,000pg/mL

Sensitivity: 5.2pg/mL

Assay Time: 2h, 40min

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Enhancer Of Zeste Homolog 2 (EZH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Enhancer Of Zeste Homolog 2 (EZH2). No significant cross-reactivity or interference between Enhancer Of Zeste Homolog 2 (EZH2) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Enhancer Of Zeste Homolog 2 (EZH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Enhancer Of Zeste Homolog 2 (EZH2) level in the sample or standard.;

Research Area: Signal transduction;

References Citing This Product: -