Features:
Effective for transfecting DNA to multiple cell lines
-High efficiency with high reproducibility
-Low toxicity to cells
-Works with medium with or without serum
-Compatible with dilution of your plasmid DNA with PBS or serum-free media, without need to change of medium during transfection.
Legend:
Lipofectamine (ThermoFisher) and ExFect (Vazyme by GeneBio Systems) were used to transfect pEGFP-C1 plasmid to HEK293、HepG-2、MCF-7、NIH3T3、HCT-116、WRL-68、A549 and HeLa cells. After 24 h, Green Fluorescent Protein expression was observed. All transfection reactions were performed in 24-well plates with plasmid used at 1 μg/ well。