EasyStep Human SAA(Serum Amyloid A) ELISA Kit

SKU: ELK042ES

Size:96T

Reactivity:Human

Alternative Names:SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Assay Type:Sandwich

Sensitivity:0.15 ng/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Infection immunity;Kidney biomarker;Hepatology;Urology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SAA, and the Human SAA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human SAA. After TMB substrate solution is added, only those wells that contain Human SAA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SAA in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P0DJI8