EasyStep Human IL1R2(Interleukin 1 Receptor Type II) ELISA Kit

SKU: ELK090ES

Size:96T

Reactivity:Human

Alternative Names:IL-1R2；CD121b; CD121-B; IL1-R2; IL1RB; Interleukin-1 Receptor Beta; CD121 Antigen-like Family Member

Assay Type:Sandwich

Sensitivity:12.7 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Signal transduction;CD & Adhesion molecule;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1R2, and the Human IL1R2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL1R2. After TMB substrate solution is added, only those wells that contain Human IL1R2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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