EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit

SKU: ELK059ES

Size:96T

Reactivity:Human

Alternative Names:BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

Assay Type:Sandwich

Sensitivity:0.98 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Metabolic pathway;Tumor immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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