EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit

SKU: ELK007ES

Size:96T

Reactivity:Human

Alternative Names:GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

Assay Type:Sandwich

Sensitivity:6.8 pg/mL

Standard:2000 pg/mL

Detection range:31.25-2000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Cardiovascular biology;Neuro science;Hormone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: