EasyStep Human aFP(Alpha-Fetoprotein) ELISA Kit

SKU: ELK091ES

Size:96T

Reactivity:Human

Alternative Names:A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein

Assay Type:Sandwich

Sensitivity:0.175 ng/mL

Standard:100 ng/mL

Detection range:1.56-100 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Tumor immunity;Hepatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aFP, and the Human aFP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human aFP. After TMB substrate solution is added, only those wells that contain Human aFP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve.

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