Dog CA125(Carbohydrate Antigen 125) ELISA Kit

SKU: ELK0153

Size:96T

Reactivity:Dog

Alternative Names:MUC16; Cancer Antigen 125; Mucin 16,Cell Surface Associated; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125

Assay Type:Sandwich

Sensitivity:0.61 U/mL

Standard:100 U/mL

Detection range:1.57-100 U/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Tumor immunity;Reproductive science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CA125. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CA125. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CA125, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CA125 in the samples is then determined by comparing the OD of the samples to the standard curve.

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